A high throughput sampler (HTS) allows automated analysis of 96 or 384 samples, each with a volume as low as 3┬ÁL.

Analysis rates of up to 10,000 events per second and minimum analysis time for a 96 well plate is 15 minutes.

Analyses include immunophenotyping, viability, DNA content, cell cycle, plant ploidy, DNA/RNA ratios, apoptosis measurements, cytokine expression, intracellular calcium, oxidative burst, intracellular pH, phagocytic activity and mitochondrial membrane potential. Flow cytometry analysis generates bivariate dot-plots and histograms which can be used to calculate population statistics and changes in mean-fluorescence intensity within the sample.

Techniques

  • Immunophenotyping, protein or cytokine expression measurements
  • DNA cell cycle and plant ploidy measurements
  • Cell viability and apoptosis analysis
  • Enzyme measurements. For example, reactive oxygen species (ROS), calcium flux, intracellular pH, mitochondrial membrane potential, or phagocytic activity
  • Marine, river or lake water phytoplankton population analysis

Instrument specifications

  • 405nm, 488nm and 633nm excitation lasers
  • Eight fluorescence parameters
  • Acquisition rate up to 10,000 events per second
  • Sampling from 96 and 384 well plates