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Flow Cytometry / Cell Sorting Equipment

Instrument

Description

Contact Info

Flow cytometer for analysis of cells, organisms or particles in suspension.  Measure relative size and complexity, cell number, viability, apoptosis, DNA content, cell cycle, DNA/RNA ratios, cytokines, intracellular calcium, oxidative burst, intracellular pH, phagocytic activity and mitochondrial membrane potential.  The HTS will run 96- and 384-well plates with a sample analysis time of approximately 15 minutes/96 well plate and a sample volume as low as 50ul.

  • 488nm and 633nm excitation lasers.
  • 6 fluorescence parameters
  • Acquisition rate up to 10000 events per second.

Room 1.42 M Block, 9346 4569 - QEII

CMCA contact: flow.cmca@uwa.edu.au

Flow cytometer for analysis of cells, organisms or particles in suspension.  Measure relative size and complexity, cell number, viability, apoptosis, DNA content, cell cycle, DNA/RNA ratios, cytokines, intracellular calcium, oxidative burst, intracellular pH, phagocytic activity and mitochondrial membrane potential.

  • 488nm and 635nm excitation lasers.
  • 4 fluorescence parameters.
  • Acquisition rate up to 2000 events per second.

Room 1.42cl, M Block, 9346 4569 - QEII

CMCA contact: flow.cmca@uwa.edu.au

Fluorescence activated cell sorter for collection of cells, organisms or particles in suspension based on degrees of expression of a trait.

  • 488nm, 635nm, 350nm excitation lasers.
  • 5 fluorescence parameters.
  • Collection of 2 populations simultaneously.
  • Sort speed up to 5000 events per second.

Room 1.40 M Block, 9346 2821 - QEII

CMCA contact: flow.cmca@uwa.edu.au

The Luminex 200 instrument is a flexible bead array system for multiplexing of up to 100 analytes per well.  The technology allows detection of soluble proteins from serum, cell supernatant and tissue homogenates, as well as oligonucleotides.  Applications include the detection of cytokines (replacing conventional ELISA), nucleic acid assays, serology isotyping and enzyme-ligand research.  Samples are run in 96-well plates with sample volumes as low as 25 µl.  The Luminex software is able to create standard curves using a number of fitting methods and automatically calculate analyte concentrations.

  • A variety of multiplexing kits available for a large range of analytes
  • Application for both protein and nucleic acid assays
  • Plate-based system able to run 96 samples and 100 analytes per hour.

Room 1.42c2, M Block, 9346 4569 - QEII

CMCA contact: flow.cmca@uwa.edu.au

Flow cytometer for analysis of cells, organisms or particles in suspension.  Measure relative size and complexity, cell number, viability, apoptosis, DNA content, cell cycle, DNA/RNA ratios, cytokines, intracellular calcium, oxidative burst, intracellular pH, phagocytic activity and mitochondrial membrane potential.

  • 488nm excitation laser.
  • 3 fluorescence parameters.
  • Acquisition rate up to 2000 events per second.

Room 556, MRF Building, 9224 0372 - QEII

CMCA contact: flow.cmca@uwa.edu.au

BD FACSCalibur Flow Cytometer
at BBCS

Flow cytometer for analysis of cells, organisms or particles in suspension.  Measure relative size and complexity, cell number, viability, apoptosis, DNA content, cell cycle, DNA/RNA ratios, cytokines, intracellular calcium, oxidative burst, intracellular pH, phagocytic activity and mitochondrial membrane potential.

  • 488nm and 635nm excitation lasers.
  • 4 fluorescence parameters.
  • Acquisition rate up to 2000 events per second.

Room 3.36, MCS Building (SBBCS), Crawley Campus.

CMCA contact: flow.cmca@uwa.edu.au
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