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Optical & Confocal Microscopes

Instrument

Description

Contact Info

Leica TCS SP2 AOBS Multiphoton Confocal

Multiphoton confocal

Inverted microscope with eight laser + multi-photon excitations. Simultaneous signal collection with 3 PMT's. FLIM attachment. Scan resolution up to 2048 x 2048 pixels. Eight laser plus multi-photon excitations are available, producing the following wavelengths: 458, 476, 488, 496, 514, 561, 594, 633, and 710-990nm (from the MP laser).

Quantitative confocal image analysis, 3D reconstruction, FRAP and FRET. A separate FLIM analysis system is also attached. Software includes 3D reconstruction with rendering, multi-color analysis, FRAP analysis, FRET analysis, animation, stereo imaging, single layer projection and quantitative analysis, time lapse collection, ratio quantification, and co-localisation analysis of 2 or 3 probes.

Room 1.74A, 6488 8063 - Crawley

CMCA contact: Dr Paul Rigby or Mr John Murphy

Zeiss Axioplan 2 Optical

Zeiss optical

Optimised for imaging via transmitted light and fluorescence. Brightfield, darkfield, Nomarski interference contrast and phase contrast imaging capabilities. Image capture via an Axiocam digital camera. Excitation wavelengths of 356nm, 488nm and 568nm.

Room 1.74 - Crawley

CMCA contact: Mr John Murphy or Mrs Lyn Kirilak

Biorad MRC 1000/1024 UV Confocal (QEII)

 

Inverted confocal microscope optimised for biological imaging and live cell analysis. The system has a motorised (computer controlled) X,Y stage and Z (focus) control for 3-dimensional imaging and time lapse, montaging and specialised software for physiological imaging and quantitation. Three fluorescence detectors and one monochrome transmission detector (with DIC imaging). Heated stage and perfusion system available.

  • Excitation laser lines: 351nm, 363.5nm, 458nm, 488nm, 514nm, 543nm, 561nm and 633nm
  • Objectives: air, water, glycerol and oil immersion; 4x – 60x; high numerical aperture.
  • Incubation chamber - temperature, CO2 and humidity control.

Room 1.42a
M Block, QEII
9346 1841

CMCA contact: Dr Paul Rigby
9346 2819

BioRad MRC1000 Confocal

Biorad confocal
Optimised to visualise fluorescent samples using confocal imaging. It may be used as an inverted or upright microscope and has objectives ranging from 5-60x plus an electronic zoom that can further increase the magnification up to 8 fold. Lasers are 488nm, 568nm and 647nm.

M Block, QEII
9346 1841

CMCA contact: Dr Paul Rigby or Mr John Murphy

Olympus IX-81 Inverted Fluorescence Microscope

Inverted fluorescence motorised microscope with high resolution DIC and phase contrast optics and equipped with a cooled FluoView II CCD digital monochrome camera. The microscope is contained within an incubation chamber controlling temperature, CO2 and humidity making it especially suitable for long-term time lapse microscopy. Water and oil immersion condensers are also available for high resolution imaging.

  • Filter cubes (narrow band): UV, blue, green and red excitation.
  • Objectives: 1x – 100x; air and oil immersion.
  • Condensers: high NA dry, water and oil immersion
  • Motorised stage
  • ZDC focus control

Room 1.21
M Block, QEII
9346 4410

CMCA Contact: Dr Paul Rigby
9346 2819

Olympus IMT-2 Inverted Fluorescence Microscope

Standard inverted fluorescence microscope with phase contrast optics. Can be equipped with Olympus DP11 digital camera, Olympus DP71 cooled CCD camera or Photonic Sciences cooled monochrome CCD camera. Heated stage available. Suitable for all routine light, phase contrast and fluorescence widefield microscopy.

  • Filter cubes: UV, violet, blue and green excitation.
  • Objectives: 1x – 100x; air and oil immersion.

Room 1.21
M Block, QEII
9346 4410

CMCA contact: Dr Paul Rigby
9346 2819

Olympus BH-2 Light Microscope

Standard upright widefield microscope. Can be equipped with Olympus DP11 digital camera, Olympus DP71 cooled CCD camera or Photonic Sciences cooled monochrome CCD camera. Suitable for all routine and high resolution widefield light microscopy and photography.


  • Objectives: 1x – 100x; air and oil immersion.
  • Darkfield condenser available

Room 1.21
M Block, QEII
9346 4410

CMCA contact: Dr Paul Rigby
9346 2819

PALM Laser Microdissector with Optical Tweezers

Laser microdissector with optical tweezers.  For collection of cell clusters, single cells or subcellular components and manipulation of cells and organisms in culture.

  • Cut and catapult at 10x, 20x, 40x, 63x and 100x magnification.
  • Suitable for FFPE sections or frozen sections.
  • Suitable for live cells.
  • Suitable for subsequent DNA/RNA/protein extraction.

Room 1.42c2
M Block, QEII
9346 4569

CMCA contact: Dr Kathy Heel
9346 4525

Aperio Scanscope Digital Slide Scanner

A robotically controlled digital slide scanner. This instrument produces high resolution transmitted light images using a 40x objective on standard microscope slides stained with H&E, DAB and other histological stains. The digital images can be viewed remotely, annotated, and captured with full control over magnification and field of view. A suite of image analysis algorithms is available for quantitative analysis, including analysis of Tissue MicroArrays (TMAs).

  • Capacity: 120 standard microscope slides (75mm x 25mm).

Room 1.21
M Block, QEII
93464410

CMCA Contact: Dr Paul Rigby
9346 2819
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