Fluorescence Spectra and Tutorials

Sources of Information for Fluorescence Spectral Data

(These sites were mostly obtained from contributions to the confocal microscopy listserver.)

1. The following site is probably the most comprehensive source of fluorescence spectra along with laser lines and different filter combinations. Created by Carl Boswell & George McNamara, it can be accessed at:
   http://www.mcb.arizona.edu/ipc/fret/ (be patient - the data set is large).

2. The original BioRad fluorochrome database can be found on the Zeiss site:
   http://www.zeiss.de/cellscience (follow the link to "fluorescent dyes").

3. Curv-o-matic at OmegaFilters: 
   http://www.omegafilters.com/

4. Fluorescence Spectrum Viewer at BD:
   http://www.bdbiosciences.com/spectra/ (intended for flow cytometry).

5. Carl Boswell's original spectra page:
   http://www.mcb.arizona.edu/IPC/spectra_page.htm

6. Invitrogen Molecular Probes:
   http://www.probes.com/servlets/spectra/

7. Jonathan Lindsey (NCSU) has a program that can graph spectra and do various calculations: PhotoChemCAD 2 at:
   http://www.photochemcad.com/

8. George McNamara's spectral data site:
   http://home.earthlink.net/~fluorescentdyes/

Fluorescence and flow cytometry tutorials

An excellent set of animated tutorials covering fluorescence, filters, light sources, flow cytometry and flow cytometry data analysis can be found on the website of Invitrogen Molecular Probes. Highly Recommended.

http://probes.invitrogen.com/resources/education/