Methods and Protocols

Specimen Preparation

Autofluorescence removal (techniques to remove or minimise autofluorescence).

Nuclear DNA staining (Hoechst 33258 and 33342, DAPI)

Mounting medium recipe (PVA and glycerol antifade mounting medium)

Using the Bio-Rad Confocal Microscope

Operating the QEII Bio-Rad Microscope - Guide to using the BioRad Confocal microscope at the QEII campus.

Confocal settings - Recommended settings for collecting images using both the Crawley and QEII Bio-Rad confocal microscopes.

Troubleshooting - Why can't I see an image?

Sample collection details form - A downloadable form for keeping track of your imaging details.

QEII UV Confocal Microscope Calibrations - Pixel sizing for scale bars for the BioRad Confocal microscope at the QEII campus.