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Sample Preparation Equipment
Instrument
| Description
| Contact Info
| | Cryopreparation suite
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High pressure freezer (Leica EM Pact2), freeze-substitution unit (Leica EM AFS2), freeze dryer (Emitech K775X Turbo) and cryomicrotome (Leica EM UC6 + cryo attachment). Provides optimal preparation of biosamples for ultrastructural, analytical and immunochemical purposes.
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Rooms 1.72 & 1.74, Crawley
CMCA contact: Dr Jeremy Shaw or Dr Peta Clode | | PELCO BioWave Microwave Processor
| Microwave preparation of biological samples for light and electron microscopy. Vacuum, stirring and temperature control capabilities. Results in accelerated and in many cases, enhanced, sample preparation.
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Room G58, 6488 8093 - Crawley
CMCA contact: Mrs Lyn Kirilak or Mr John Murphy | PALM Laser Microdissector with Optical Tweezers
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Laser microdissector with optical tweezers. For collection of cell clusters, single cells or subcellular components and manipulation of cells and organisms in culture. - Cut and catapult at 10x, 20x, 40x, 63x and 100x magnification.
- Suitable for FFPE sections or frozen sections.
- Suitable for live cells.
- Suitable for subsequent DNA/RNA/protein extraction.
| Room 1.42c2 M Block, 9346 4569 - QEII CMCA contact: Dr Kathy Heel | | Vibratome 3000
| Deluxe EM vibratome tissue sectioning system with temperature control and various stage assemblies. For micron-scale sectioning of fresh, fixed or agarose embedded materials.
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Room 1.72, 6488 8061 - Crawley
CMCA contact: Mr John Murphy or Mrs Lyn Kirilak | | Critical Point Dryer
| For preparation of biological samples for SEM. Uses carbon dioxide and has a wide range of specimen baskets
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Room G58, 6488 8093 - Crawley
CMCA contact: Mr John Murphy or Mrs Lyn Kirilak
| | Microtomes
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A new Leica EM UC6 microtome (with cryo attachment), Reichert ultramicrotome and a Sorval microtome; for cutting TEM and light microscopy sections (30nm - 5um).
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Room 1.72, 6488 8061 - Crawley
CMCA contact: Mr John Murphy or Mrs Lyn Kirilak
| | PIPS
| Preparation of thin TEM samples in materials sciences
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Room G73, 6488 8081 - Crawley
CMCA contact: Dr Martin Saunders or Dr Alexandra Suvorova | | Ultrasonic Disc Cutter
| Preparation of thin TEM samples for materials sciences
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Crawley
CMCA contact: Dr Martin Saunders or Dr Alexandra Suvorova
| | Polishing equipment
| Buehler Handimet® 2 roll grinder for manual grinding applications. Self contained 4-stage grinding station with controlled water flow.
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Room G72, 6488 8094 - Crawley
CMCA contact: Mr Steve Parry | | Vacuum oven
| Aids resin infiltration and curing under vacuum. Can be used at room temperature and as a curing oven.
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Room G58, 6488 8093 - Crawley
CMCA contact: Mr John Murphy or Mrs Lyn Kirilak |
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