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Sample Preparation Equipment

Instrument

Description

Contact Info

Cryopreparation suite
High pressure freezer (Leica EM Pact2), freeze-substitution unit (Leica EM AFS2), freeze dryer (Emitech K775X Turbo) and cryomicrotome (Leica EM UC6 + cryo attachment). Provides optimal preparation of biosamples for ultrastructural, analytical and immunochemical purposes.

Rooms 1.72 & 1.74, Crawley

CMCA contact: Dr Jeremy Shaw or Dr Peta Clode

PELCO BioWave Microwave Processor
Microwave preparation of biological samples for light and electron microscopy.  Vacuum, stirring and temperature control capabilities. Results in accelerated and in many cases, enhanced, sample preparation.

Room G58, 6488 8093 - Crawley

CMCA contact: Mrs Lyn Kirilak or Mr John Murphy

PALM Laser Microdissector with Optical Tweezers
Laser microdissector with optical tweezers. For collection of cell clusters, single cells or subcellular components and manipulation of cells and organisms in culture.
  • Cut and catapult at 10x, 20x, 40x, 63x and 100x magnification.
  • Suitable for FFPE sections or frozen sections.
  • Suitable for live cells.
  • Suitable for subsequent DNA/RNA/protein extraction.

Room 1.42c2 M Block, 9346 4569 - QEII

CMCA contact: Dr Kathy Heel

Vibratome 3000
Deluxe EM vibratome tissue sectioning system with temperature control and various stage assemblies. For micron-scale sectioning of fresh, fixed or agarose embedded materials.

Room 1.72, 6488 8061 - Crawley

CMCA contact: Mr John Murphy or Mrs Lyn Kirilak

Critical Point Dryer
For preparation of biological samples for SEM. Uses carbon dioxide and has a wide range of specimen baskets
Room G58, 6488 8093 - Crawley

CMCA contact: Mr John Murphy or Mrs Lyn Kirilak

Microtomes
A new Leica EM UC6 microtome (with cryo attachment), Reichert ultramicrotome and a Sorval microtome; for cutting TEM and light microscopy sections (30nm - 5um).
Room 1.72, 6488 8061 - Crawley

CMCA contact: Mr John Murphy or Mrs Lyn Kirilak

PIPS
Preparation of thin TEM samples in materials sciences

Room G73, 6488 8081 - Crawley

CMCA contact: Dr Martin Saunders or Dr Alexandra Suvorova

Ultrasonic Disc Cutter
Preparation of thin TEM samples for materials sciences

Crawley

CMCA contact: Dr Martin Saunders or Dr Alexandra Suvorova

Polishing equipment
Buehler Handimet® 2 roll grinder for manual grinding applications. Self contained 4-stage grinding station with controlled water flow.

Room G72, 6488 8094 - Crawley

CMCA contact: Mr Steve Parry

Vacuum oven
Aids resin infiltration and curing under vacuum. Can be used at room temperature and as a curing oven.

Room G58, 6488 8093 - Crawley

CMCA contact: Mr John Murphy or Mrs Lyn Kirilak

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