CMCA facilities are located on four separate sites including the third floor of the Indian Ocean Marine Research Centre (IOMRC) building on the Crawley campus. Access to these facilities is by UWA campus card.

Located on the Third Floor

BD FACSCalibur

The FACSCalibur analyses cells or particles at a rate of up to 2000 events per second, a high throughput sampler (HTS) allows for automated sampling from 96 or 384 well plates.

Analyses include immunophenotyping, viability, DNA content, cell cycle, plant ploidy, DNA/RNA ratios, apoptosis measurements, cytokine expression, intracellular calcium, oxidative burst, intracellular pH, phagocytic activity, mitochondrial membrane potential and bead array assays.

Flow cytometry analysis generates bivariate dot-plots and histograms which can be used to calculate population statistics and changes in mean-fluorescence intensity within the sample.

Techniques
  • Immunophenotyping, protein or cytokine expression measurements
  • DNA cell cycle and ploidy measurements
  • Cell viability and apoptosis analysis
  • Enzyme measurements. For example, reactive oxygen species (ROS), calcium flux, intracellular pH, mitochondrial membrane potential or phagocytic activity
  • Cytometric bead array (CBA) or flex arrays
  • Marine, river or lake water phytoplankton population analysis
Instrument specifications
  • 488nm and 635nm excitation lasers
  • Four fluorescence parameters
  • Acquisition rate up to 2000 events per second
  • The QEII instrument has a high throughput sampler (HTS) allowing sampling from 96 and 384 well plates

Leica TCS SP2 multiphoton confocal microscope

The Leica is an inverted microscope with eight laser and multi-photon excitations.

The system is capable of simultaneous image collection, quantitative confocal image analysis, 3D reconstruction and most multiphoton microscopy techniques. A separate Becker & Hickle Fluorescence Loss Imaging Microscopy (FLIM) analysis system is also attached.

Software includes 3D reconstruction with rendering, multi-colour analysis, fluorescence recovery after photobleaching (FRAP) analysis, fluorescence resonance energy transfer (FRET) analysis, animation, stereo imaging, single layer projection and quantitative analysis, time-lapse collection, ratio quantification and co-localisation analysis of two or three probes.

Techniques
  • Four colour confocal microscopy
  • Multiphoton microscopy including:
    • Fluorescence recovery after photobleaching (FRAP)
    • Fluorescence resonance energy transfer (FRET)
    • Secondary harmonic generation (SHG)
    • Fluorescence loss in photobleaching (FLIP)
    • Fluorescence correlation spectroscopy (FCS)
Instrument specifications
  • Objectives 1.6x-100x, air, water and oil immersion
  • Laser wavelengths: 458, 476, 488, 496, 514, 561, 594, 633
  • Spectra-physics Mai-Tai multi-photon laser tuneable from 710-990nm
  • Four photo multiplier tube (PMT) detectors
  • Confocal laser differential interference contrast (DIC) and phase imaging
  • Oil condenser lens for SHG
  • Scan resolution up to 2048 x 2048 pixels
Publishing data

Access and rates

To view the full range of CMCA access options and costs see:

Access to IOMRC for CMCA registered users

The Leica MP Confocal microscope and the BD Calibur Cytometer are located on the third floor inside AIMS.

Users must be registered with CMCA and have a current subscription.

If you are a UWA student or staff member you will need to:
  1. Login to http://www.lms.uwa.edu.au using you Pheme credentials
  2. Click on the Units tab at the top of the page
  3. In the Unit Search box, type in “IOMRC” to display the units
  4. Mouse-over the units, click on the drop-down arrow that appears and click “Enrol”
  5. You will see two modules:
    1. IOMRC Crawley ‐ Building Induction
    2. IOMRC-Crawley ‐ Laboratory-Induction
  6. Please complete both modules and send your certificate to Admin at CMCA (admin-cmca@uwa.edu.au). Swipe card access will then be arranged.
  7. Please contact the TGL responsible for the instruments for a walk-around inside IOMRC.
  8. All instruments will need to be booked on ACLS prior to use
If you are a non-UWA student or staff member you will need to:
  1. Obtain a UWA visitors card from the student centre
  2. Register your Pheme account
  3. Login to http://www.lms.uwa.edu.au Using your Pheme credentials and click “Guest”
  4. Click on the Units tab at the top of the page
  5. In the Unit Search box, type in “IOMRC” to display the units
  6. Mouse-over the units, click on the drop-down arrow that appears and click “Enrol”
  7. You will see two modules:
    1. IOMRC Crawley - Building Induction
    2. IOMRC-Crawley - Laboratory-Induction
  8. Please complete both modules and send your certificate to Admin at CMCA (admin-cmca@uwa.edu.au). Swipe card access will then be activated.
  9. Please contact the TGL responsible for the instruments for a walk-around inside IOMRC.
  10. All instruments will need to be booked on ACLS prior to use.

Publication and acknowledgement

An acknowledgment to CMCA staff (this is considered a KPI for staff and is reported):

  • Personally acknowledge CMCA staff who have helped you in your activities
  • Have CMCA staff helped you beyond simple instrument operation? If so, consider including them as authors on your publication

An acknowledgment to CMCA:

'The authors acknowledge the facilities, and the scientific and technical assistance of the Australian Microscopy and Microanalysis Research Facility at the Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, a facility funded by the University, State and Commonwealth Governments.'

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