Through two lectures and two to three practical sessions, you will be trained in basic operations of the transmission electron microscopes (TEM).
Who can enrol
Researchers, PhD and honours students intending to use the CMCA's TEM facilities can undertake the course.
The main instrument operation and alignment steps, image interpretation and applications of TEM will be introduced through lectures. You will receive two or three training sessions on the microscope.
Where possible, training will be conducted in pairs.
You will learn basic TEM operation skills including:
- carrying out standard microscope alignments
- conventional imaging, for example, obtaining shape, size and structural information at magnifications up to ~100kx
- interpreting images (mass-thickness contrast)
- recording images with digital cameras
- basic sample requirements for TEM (but not sample preparation methods).
Not included in the course are more advanced topics such as electron diffraction, high-resolution (crystal lattice) imaging, composition analysis (via energy-dispersive X-ray microanalysis or electron energy-loss spectroscopy), element distribution imaging (via energy-filtered TEM) or scanning TEM imaging.
Detailed information on sample preparation techniques in the physical and biological sciences is not included.
- Lectures: 3-4 hours
- Laboratories: Two or three 90-minute, hands-on lab sessions
- Laboratory times will be advised approximately one week before the course.
Copies of lecture notes and supporting documentation are provided as a downloadable file available one week before the course starts. You are expected to be familiar with basic principles and applications of TEM before attending the course. Many TEM-related books can be found in the UWA library.
- Transmission electron microscopy (1996), D.B. Williams and C.B. Carter, Plenum Press (Physical Sciences)
- Electron microscopy: Principles and techniques for biologists, Bozzola and Russell (Biological Sciences)
You are expected to be familiar with the basic principles and applications of TEM and the TEM aspects of your research project before attending the course.
Practical assessment will take place during the training laboratories on the instruments.
A Centre staff member will sit in on your first TEM session after the course to provide guidance and assess whether you need additional training.