The Caliper IVIS Lumina II enables real-time, non-invasive visualisation and tracking of fluorescent or bioluminescent cells, proteins or gene activity within a living animal. It can also be used to visualise dishes, plates and tissue samples.

Caliper IVIS Lumina II

The system integrates a highly sensitive charge-coupled device (CCD) cooled camera capable of detecting exceptionally low light levels, with a light-tight imaging chamber and includes a broad range of filter sets that permits imaging of a wide range of fluorophores.

Techniques

  • In vivo fluorescence imaging
  • In vivo bioluminescence imaging
  • Autofluorescence correction

Instrument specifications

  • 16-bit thermoelectrically cooled CCD camera (cooled to -90oC)
  • Minimum image pixel resolution 50 ┬Ám
  • Field of view 5 cm x 5 cm to 12.5 cm x 12.5 cm (variable zoom)
  • Heated stage to maintain optimal body temperature
  • Anaesthesia manifold for anaesthesia induction
  • Animal isolation containment chambers for up to three mice or one rat
  • 10 excitation and four broad emission fluorescence filter sets
  • Living image software

Operating guides

Lumina Filter Sets [PDF, 24.8 KB]
Updated 13 Feb 2013


Publishing data

Further Information


Access

Location

Contacts

CMCA rates

Animal Care

The Bioimaging Facility is committed to maintaining the highest standards of animal care and welfare. We operate in collaboration with Animal Care Services who provide pre and post imaging care.

All animal imaging procedures require approval from the UWA Animal Ethics Committee which incorporates a pre-submission discussion with both Animal Care and Veterinary Services and Bioimaging Facility staff.