The FACSCalibur analyses cells or particles at a rate of up to 2000 events per second, a high throughput sampler (HTS) allows for automated sampling from 96 or 384 well plates.

Analyses include immunophenotyping, viability, DNA content, cell cycle, plant ploidy, DNA/RNA ratios, apoptosis measurements, cytokine expression, intracellular calcium, oxidative burst, intracellular pH, phagocytic activity, mitochondrial membrane potential and bead array assays.

Flow cytometry analysis generates bivariate dot-plots and histograms which can be used to calculate population statistics and changes in mean-fluorescence intensity within the sample.


  • Immunophenotyping, protein or cytokine expression measurements
  • DNA cell cycle and ploidy measurements
  • Cell viability and apoptosis analysis
  • Enzyme measurements. For example, reactive oxygen species (ROS), calcium flux, intracellular pH, mitochondrial membrane potential or phagocytic activity
  • Cytometric bead array (CBA) or flex arrays
  • Marine, river or lake water phytoplankton population analysis

Instrument specifications

  • 488nm and 635nm excitation lasers
  • Four fluorescence parameters
  • Acquisition rate up to 2000 events per second
  • The QEII instrument has a high throughput sampler (HTS) allowing sampling from 96 and 384 well plates

Further Information