The Leica is an inverted microscope with eight laser and multi-photon excitations.

The system is capable of simultaneous image collection, quantitative confocal image analysis, 3D reconstruction and most multiphoton microscopy techniques. A separate Becker & Hickle Fluorescence Loss Imaging Microscopy (FLIM) analysis system is also attached.

Software includes 3D reconstruction with rendering, multi-colour analysis, fluorescence recovery after photobleaching (FRAP) analysis, fluorescence resonance energy transfer (FRET) analysis, animation, stereo imaging, single layer projection and quantitative analysis, time-lapse collection, ratio quantification and co-localisation analysis of two or three probes.

Techniques

  • Four colour confocal microscopy
  • Multiphoton microscopy including:
    • Fluorescence recovery after photobleaching (FRAP)
    • Fluorescence resonance energy transfer (FRET)
    • Secondary harmonic generation (SHG)
    • Fluorescence loss in photobleaching (FLIP)
    • Fluorescence correlation spectroscopy (FCS)

Instrument specifications

  • Objectives 1.6x-100x, air, water and oil immersion
  • Laser wavelengths: 458, 476, 488, 496, 514, 561, 594, 633
  • Spectra-physics Mai-Tai multi-photon laser tuneable from 710-990nm
  • Four photo multiplier tube (PMT) detectors
  • Confocal laser differential interference contrast (DIC) and phase imaging
  • Oil condenser lens for SHG
  • Scan resolution up to 2048 x 2048 pixels

Publishing data