The Leica is an inverted microscope with eight laser and multi-photon excitations.
The system is capable of simultaneous image collection, quantitative confocal image analysis, 3D reconstruction and most multiphoton microscopy techniques. A separate Becker & Hickle Fluorescence Loss Imaging Microscopy (FLIM) analysis system is also attached.
Software includes 3D reconstruction with rendering, multi-colour analysis, fluorescence recovery after photobleaching (FRAP) analysis, fluorescence resonance energy transfer (FRET) analysis, animation, stereo imaging, single layer projection and quantitative analysis, time-lapse collection, ratio quantification and co-localisation analysis of two or three probes.
- Four colour confocal microscopy
- Multiphoton microscopy including:
- Fluorescence recovery after photobleaching (FRAP)
- Fluorescence resonance energy transfer (FRET)
- Secondary harmonic generation (SHG)
- Fluorescence loss in photobleaching (FLIP)
- Fluorescence correlation spectroscopy (FCS)
- Objectives 1.6x-100x, air, water and oil immersion
- Laser wavelengths: 458, 476, 488, 496, 514, 561, 594, 633
- Spectra-physics Mai-Tai multi-photon laser tuneable from 710-990nm
- Four photo multiplier tube (PMT) detectors
- Confocal laser differential interference contrast (DIC) and phase imaging
- Oil condenser lens for SHG
- Scan resolution up to 2048 x 2048 pixels