Nikon A1RMP confocal and multiphoton microscope
The system is capable of simultaneous or sequential image collection using galvanometer and resonant scanners and four photomultiplier fluorescence detectors and a DIC transmission detector. The multiphoton components comprise a Coherent Chameleon II pulsed laser (700nm-1000nm), NDD detectors and the Nikon Apo 25x WMP dipping objective. An objective inverter will allow an effective upright configuration for the Nikon Ti E (inverted) microscope which is suitable for in vivo animal imaging.
Techniques
- Four colour confocal microscopy
- Multipoint and live time-lapse imaging
- Multiphoton imaging
- High-speed resonant scanning
- Transmitted light DIC imaging
- In vivo imaging using objective inverter and dipping objectives
Instrument specifications
- Inverted Nikon Ti E microscope
- Nikon PlanApo objectives 10x-100x, air, water and oil immersion
- Four 20mW solid state lasers – 405nm (violet), 488nm (blue), 561nm (yellow), and 638nm (red).
- Coherent Chameleon II infrared laser (700nm-1000nm)
- Galvanometer and Resonant scanners
- Nikon Apo 25x Water MultiPhoton objective
- Four PMT detectors
- Four NDD (reflected light) detectors
- NIS Elements software controlling all microscope components
- LSMTech objective inverter and external stage
- Perfect focus system (PFS)
Publishing data
