The WA Proteomics Facility is a node of Proteomics Australia based at CMCA that provides services to WA researchers including - sample analysis by mass spectrometry, data analysis and consulting. Services are delivered through a joint venture between The University of Western Australia and Proteomics International.

Our goal is to use our expertise and equipment to fuel cutting-edge research. We strive to form strong collaborations with any interested scientists to increase the value of their research.

Services are focused on protein identification, quantitation and flux analysis.

What is Proteomics?

Proteomics is the large-scale study of proteomes. A proteome is a set of proteins produced in an organism, system, or biological context. The proteome is not constant; it differs from cell to cell, tissue to tissue and it changes over time. While the proteome relies on the underlying transcriptome for its synthesis, protein abundance and activity is also modulated by many factors in addition to mRNA abundance. Protein abundance is poorly predicted by transcript abundance in many contexts making such data independently valuable and highly complementary when analysing complex biological questions.

In general terms, proteomic techniques determine:

  • when and where proteins are expressed
  • rates of protein production, degradation, and steady-state abundance
  • how proteins are modified (for example, post-translational modifications (PTMs) such as phosphorylation)
  • the involvement of proteins in metabolic pathways
  • how proteins interact with one another

The services offered by the WA Proteomics Facility focus on:

  • Protein identification to provide evidence to link a protein to the gene that encodes it
  • Quantitative proteomics to reveal the abundance of specific proteins in a complex sample
  • Targeted proteomics to enable specific proteins of interest to be analysed
  • Flux proteomics to measure the synthesis and degradation rates of proteins

Instruments

ThermoFisher Orbitrap Fusion and Exploris 480 Instruments are some of the most powerful available for delivering quantitative proteomics and flux proteomics services. Triple quadrupole instruments are used for targeted proteomics services. This facility is located in Room G28, Bayliss Building, UWA Perth campus.

If you want to understand more about proteomics and how it can relate to your biological question, contact [email protected].