The Caliper IVIS Lumina II enables real-time, non-invasive visualisation and tracking of fluorescent or bioluminescent cells, proteins or gene activity within a living animal. It can also be used to visualise dishes, plates and tissue samples.
The system integrates a highly sensitive charge-coupled device (CCD) cooled camera capable of detecting exceptionally low light levels, with a light-tight imaging chamber and includes a broad range of filter sets that permits imaging of a wide range of fluorophores.
Techniques
- In vivo fluorescence imaging
- In vivo bioluminescence imaging
- Autofluorescence correction
Instrument specifications
- 16-bit thermoelectrically cooled CCD camera (cooled to -90oC)
- Minimum image pixel resolution 50 µm
- Field of view 5 cm x 5 cm to 12.5 cm x 12.5 cm (variable zoom)
- Heated stage to maintain optimal body temperature
- Anaesthesia manifold for anaesthesia induction
- Animal isolation containment chambers for up to three mice or one rat
- 10 excitation and four broad emission fluorescence filter sets
- Living image software
Operating guides
Publishing data
Further Information
Location
- Level 3, Harry Perkins Institute of Medical Research, QEII Medical Centre, 6 Verdun Street, Nedlands
Contacts
CMCA rates
- Rates fall under standard CMCA access.
Animal Care
The Bioimaging Facility is committed to maintaining the highest standards of animal care and welfare. We operate in collaboration with Animal Care Services who provide pre and post imaging care.
All animal imaging procedures require approval from the UWA Animal Ethics Committee which incorporates a pre-submission discussion with both Animal Care and Veterinary Services and Bioimaging Facility staff.