Cutting edge proteomics services delivered through our world class expertise and state of the art instrumentation
Proteins lie at the heart of all biology. We offer cutting edge services for the analysis, identification and quantitation of proteins as well as specialist services in measuring protein synthesis and degradation rates. At Proteomics Western Australia we offer both deep expertise and a user friendly environment to help you design and execute your studies successfully while we deliver world class data and analysis on WA's state of the art infrastructure. Our enduring aim is to build successful collaborations with the life sciences sector to contribute to Australia’s health, environmental, economic and social wellbeing. We can also offer support to researchers in the form of grant writing assistance and utilisation of the facility generally qualifies as an in-kind contribution from NCRIS and Bio Platforms Australia, helping your grant applications be the most competitive they can.
The WA Proteomics Facility is a node of Proteomics Australia based at CMCA. Services are delivered through a joint venture between The University of Western Australia and Proteomics International.
The services offered by the WA Proteomics Facility focus on:
- Protein identification to provide evidence to link protein to the gene that encodes it
- Label free quantitation to reveal the identity and abundance of proteins in a complex sample
- Targeted proteomics to selectively quantitate specific proteins of interest
- Flux proteomics to measure the synthesis and degradation rates of proteins
Label Free Quantitative Proteomics
Our protein identification service offers a cut price entry to the world of proteomics and is suitable for routine protein identification and sample quality control tasks. Analysed on a short gradient on one of our Orbitrap instruments, this service can provide an extensive list of the protein(s) present in a sample and make estimates as to the relative proportion each protein constitutes of the total sample.
Our targeted proteomics service allows researchers to track the abundance of a smaller number of specific proteins across multiple samples. This analysis has the advantage of allowing the highest confidence measurements of relative abundance available in proteomics as well as the ability to show that the specified proteins are absent (below the limit of detection of the instrumentation). This technique is commonly described as a mass Western but is much faster, more quantitative, not prone to cross reactivity and can generally be tailored to specific isoforms of proteins. This service includes sample digestion, cleanup and mass spec analysis followed by data processing to supply a spreadsheet of proteins present and their normalised relative abundances across samples.
Protein flux analysis offers the opportunity to understand how much of which proteins are being translated and degraded in your experimental system. These measurements allow a much more detailed understanding of the dynamics of protein biology than simple abundance measurements. In this methodology a pulse of stable isotope labeled substrate is fed into the experimental system which is metabolically incorporated into the amino acid pool and subsequently into newly synthesised protein. By tracking the rate of accumulation of protein with incorporated stable isotopes relative to the preexisting unlabelled protein, both the rate of translation and degradation can be derived from a set of samples. This service includes help to design the labelling strategy, analyse the samples and process data to supply relative synthesis and degradation rates for abundant proteins in your samples.
Thermo Fisher Orbitrap Fusion and Exploris 480 Instruments are some of the most powerful available for delivering quantitative proteomics and flux proteomics services. Triple quadrupole instruments are used for targeted proteomics services. This facility is located in Room G28, Bayliss Building, UWA Perth campus.
If you want to understand more about proteomics and how it can relate to your biological questions, contact [email protected]
Meet the team
With several decades of dedicated proteomics research combined, our scientists can help you get the most out of your experiment. The WA proteomics team at UWA consists of Dr Elke Stroeher (left), our Operations Manager, Professor Harvey Millar (centre) our Platform Lead and Dr Owen Duncan (right), our Scientific Lead.