The CRi Maestro 2 is a high-performance multispectral imaging system designed for fluorescence macro-imaging of small animals such as a mice or rats.

CRi Maestro 2

The system is equipped with unique multispectral tunable optics and inbuilt multispectral imaging software algorithms which enables users to rapidly image and quantify more than one fluorescent signal within a mouse or rat in real time.

The system can be used for multiplexing and spectral unmixing enabling imaging of multiple fluorescent signals simultaneously together with the removal of any autofluorescence from an image.


  • In vivo fluorescence imaging
  • In vivo multispectral imaging and unmixing
  • Auto fluorescence correction

Instrument specifications

  • 12-bit CCD imaging sensor
  • Minimum image pixel resolution 25 ┬Ám
  • Field of view (length x width): 3.9 cm x 2.9 cm to 11.2 cm x 8.4 cm (variable zoom)
  • Heated imaging chamber and stage to maintain optimal body temperature.
  • Anaesthesia manifold for anaesthesia induction
  • Animal isolation containment chambers for up to three mice or one rat
  • 8 excitation filter sets
  • Solid-state liquid crystal tuneable filter
  • Wavelength tuning range 500-950 nm
  • Maestro software including CPSTM (Compute Pure Spectrum) and RCATM (Real Component Analysis) spectral library generation tools

Operating guides

Publishing data

Further Information



CMCA rates

Animal Care

The NIF Bioimaging Facility is committed to maintaining the highest standards of animal care and welfare. We operate in collaboration with Animal Care Services who provide pre and post imaging care.

All animal imaging procedures require approval from the UWA Animal Ethics Committee which incorporates a pre-submission discussion with both Animal Care and Veterinary Services and Bioimaging Facility staff.